Journal: Kidney international

Article Title: Early treatment with cGMP phosphodiesterase inhibitor ameliorates progression of renal damage.

doi: 10.1111/j.1523-1755.2005.00669.x

Figure Lengend Snippet: Fig. 6. Staining with panendothelial monoclonal antibody (RECA-1) expressed as the ratio of positive area/total area in glomeruli (A) and tubulointerstitial regions (B). Renal ablation [5/6 nephrectomy (5/6 Nx)] reduces and the treatment with sildenafil (5/6 Nx.SIL) prevents the loss of glomerular and peritubular capillaries. ∗P < 0.05; ∗∗∗P < 0.001. Representative microphotographs of renal sections of a vehicle-treated rat (5/6 nephrectomy group) (C) and a sildenafil-treated rat (D) (immunoperoxidase staining).

Article Snippet: Glomerular and peritubular capillaries were identified with panendothelial monoclonal mouse antirat RECA-1 antibody (clone HIS52) (Serotec Inc., Raleigh, NC, USA).

Techniques: Staining, Immunoperoxidase Staining

Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Article Title: Donor and recipient origin of mesenchymal and endothelial cells in chronic renal allograft remodeling.

doi: 10.1111/j.1600-6143.2008.02534.x

Figure Lengend Snippet: Figure 4: Graft origin of neointimal SMC and ECs. (A–D) Graft origin of neointimal SMCs in a DA-to-R26(2.5) allograft. Sections were stained for a-SMA (red, panel A), hPAP (green, panel B) and DA MHC class I (blue, panel C). (D) The merged image shows graft-derived neointimal SMCs that express DA MHC class I but not hPAP. Magnification 3150×. (E–H) Graft origin of ECs in arteries. (E) Coexpression of hPAP (red) and RECA-1 (EC, green) in arterial ECs in an R26 kidney. (F) Serial section of E after double labeling for hPAP (red) and DA MHC class I (green). Magnification 1260×. (G) Artery with neointima in a DA-to-R26(2.5) allograft showing neointimal ECs negative for hPAP. (H) Serial section of panel (G) showing that the ECs are positive for DA MHC class I. Magnification 2520×. Dotted line in panels (A–D), (G) and (H) indicates the internal elastic lamina (IEL).

Article Snippet: The sections were incubated for 1 h with the following primary antibodies: a-SMA, mouse anti-rat endothelium (RECA-1) (15), mouse-antirat major histocompatibility complex (MHC) class I RT1Aa (DA MHC class I, clone MN4-91-6; AbD Serotec, Oxford, UK), rabbit anti-hPAP polyclonal antibody (AbD Serotec) and goat anti-procollagen I (Santa Cruz Biotechnology, Santa Cruz, CA). a-SMA was detected using goat anti-mouse IgG2aHRP.

Techniques: Staining, Derivative Assay, Labeling

Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Article Title: Donor and recipient origin of mesenchymal and endothelial cells in chronic renal allograft remodeling.

doi: 10.1111/j.1600-6143.2008.02534.x

Figure Lengend Snippet: Figure 5: Recipient EC chimerism in glomerular capillaries. (A) Glomerulus in a nontransplanted R26 kidney double-labeled for hPAP (green) and RECA-1 (EC, red). (B) Glomerulus in a DA kidney double- labeled for hPAP (green) and RECA-1 (EC, red). Magnification 2520×. (C) Glomerulus in a DA-to-R26(5) allograft with recipient-derived ECs identified by coexpression of both hPAP (red) and RECA-1 (EC, green). Magni- fication 1260×. (D) Higher-power magnification of the framed area shown in panel (C). The x/z plane underneath and the y/z plane on the right demonstrate colocalization of hPAP and RECA-1. Magnification 4410×.

Article Snippet: The sections were incubated for 1 h with the following primary antibodies: a-SMA, mouse anti-rat endothelium (RECA-1) (15), mouse-antirat major histocompatibility complex (MHC) class I RT1Aa (DA MHC class I, clone MN4-91-6; AbD Serotec, Oxford, UK), rabbit anti-hPAP polyclonal antibody (AbD Serotec) and goat anti-procollagen I (Santa Cruz Biotechnology, Santa Cruz, CA). a-SMA was detected using goat anti-mouse IgG2aHRP.

Techniques: Labeling, Derivative Assay

Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Article Title: Donor and recipient origin of mesenchymal and endothelial cells in chronic renal allograft remodeling.

doi: 10.1111/j.1600-6143.2008.02534.x

Figure Lengend Snippet: Figure 6: Graft and recipient origin of interstitial myofibroblasts. (A) Rep- resentative photomicrograph of cortical interstitium in a DA-to-R26(5) allograft stained for a-SMA (red), hPAP (green) and DA MHC class I (blue) showing a- SMA+/DA MHC class I+ graft-derived myofibroblasts. Magnification 1260×. (B) Higher-power magnification (3150×) of the framed area shown in panel (A). Arrow indicates an a-SMA+/DA MHC class I+ graft-derived myofibro- blast. (C) Identical section and stain- ing as shown in panel (A) now depict- ing a-SMA+/hPAP+ recipient-derived myofibroblasts. Magnification 1260×. (D) Higher-power magnification (3150×) of the framed area shown in panel (C). Arrows indicate a-SMA+/hPAP+

Article Snippet: The sections were incubated for 1 h with the following primary antibodies: a-SMA, mouse anti-rat endothelium (RECA-1) (15), mouse-antirat major histocompatibility complex (MHC) class I RT1Aa (DA MHC class I, clone MN4-91-6; AbD Serotec, Oxford, UK), rabbit anti-hPAP polyclonal antibody (AbD Serotec) and goat anti-procollagen I (Santa Cruz Biotechnology, Santa Cruz, CA). a-SMA was detected using goat anti-mouse IgG2aHRP.

Techniques: Staining, Derivative Assay

Journal: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Article Title: Donor and recipient origin of mesenchymal and endothelial cells in chronic renal allograft remodeling.

doi: 10.1111/j.1600-6143.2008.02534.x

Figure Lengend Snippet: Figure 7. Procollagen I expression in recipient-derived myofibroblast. Sec- tions were stained for a-SMA (red, A), procollagen I (green, B) and hPAP (blue, C). Figure D shows the merged image of panels (A–C). Arrows indicate a procolla- gen I-expressing recipient-derived inter- stitial myofibroblast in a DA-to-R26(5) al- lograft. Magnification 640×.

Article Snippet: The sections were incubated for 1 h with the following primary antibodies: a-SMA, mouse anti-rat endothelium (RECA-1) (15), mouse-antirat major histocompatibility complex (MHC) class I RT1Aa (DA MHC class I, clone MN4-91-6; AbD Serotec, Oxford, UK), rabbit anti-hPAP polyclonal antibody (AbD Serotec) and goat anti-procollagen I (Santa Cruz Biotechnology, Santa Cruz, CA). a-SMA was detected using goat anti-mouse IgG2aHRP.

Techniques: Expressing, Derivative Assay, Staining

Journal: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association

Article Title: Angiotensin receptor blockade attenuates glomerulosclerosis progression by promoting VEGF expression and bone marrow-derived cells recruitment.

doi: 10.1093/ndt/gfr621

Figure Lengend Snippet: Fig. 4. Valsartan administration promoted BMDCs recruitment that nearly all expressed the monocyte/macrophage marker positive. The representative confocal images of BMDCs recruitment into glomeruli from either untreated rat (A) or ARB treatment (B) on Day 56 after injection of 1-22-3 antibody. (C) Graph showing mean number of BMDCs in glomeruli of two groups. *P < 0.05 versus untreated group. The representative confocal images of immunofluorescent staining for monocytes/macrophages (CD68) with glomerular sections from either untreated rat (D) or ARB treatment (E) on Day 56 after injection of 1-22-3 antibody. (F) Graph showing the percentage of BM-derived monocytes/macrophages (CD68 + ), CD45-positive cells and CD45-negative cells both untreated and ARB group. The representative confocal images of immunofluorescent staining for leukocytes (CD45) with glomerular sections from untreated group (G) or ARB treatment group (H) on Day 56 after injection of 1-22-3 antibody. BMDCs with CD45—marked with arrows.

Article Snippet: The primary antibodies were mouse anti-rat OX-7 antibody (A hybridoma producing OX-7 IgG1 was purchased from European Collection of Animal Cells Porton Down, Salisbury, UK) for identifying glomerular mesangial cells and mouse anti-rat RECA-1 mouse antibody (Serotec Ltd, Kindlington, UK) for identifying glomerular endothelial cells (GECs) and rabbit antirat ZO-1 antibody (Zymed Laboratories, San Francisco, CA) for identifying podocyte, mouse anti-rat CD45 (Abcam, Cambridge, UK) antibody for identifying leukocyte, mouse anti-rat CD68 (Serotec Ltd) antibody for identifying monocyte/macrophage and mouse anti-vascular endothelial growth factor (VEGF) monoclonal antibody (Abcam).

Techniques: Marker, Injection, Staining, Derivative Assay